Past Lab Members

  C. Hart Poskar   

Hart’s research at MSU focused on automation and network modeling. While at MSU Hart’s motto was: “It’s never too cold for a trip to the MSU Dairy Store.”

.

.

.

Jay Allen

Ph.D. student, received 2009

Allen JW, Shachar-Hill Y (2009) Sulfur transfer through an Arbuscular Mycorrhiza. Plant Physiology 149: 549-560  PDF

 .

.

 Russel LaClair

Research Technician II, 2008-2009

His research was focused on two GLBRC projects that quantified cell wall metabolites using LC-MS/MS and using Rubisco as a model protein for plant compartmentalization.

.

.

Doug Allen  

Doug’s work in the group focused on the development of metabolic flux analysis tools and their application to developing seeds.  His methods yield more accurate information on flux through key inetermediates and new insight into the subcellular compartmentation of metabolic fluxes. Doug’s in depth study of developing soy beans  revealed the roles of light and different metabolic precursors in the synthesis of the economically important soy oil and protein. Doug joined the Donald Danforth Plant Science Research Center in St Louis as a group leader in 2010

Allen DK, Libourel IGL, Shachar-Hill Y (2009) Metabolic flux analysis in plants: coping with complexity. Plant Cell and …………………   …… Environment. 32: 1241-1257  PDF                                                                                                                                                …………………………Allen DK, Ohlrogge JB, Shachar-Hill Y (2009) The role of light in soybean seed filling metabolism. Plant Journal. 58:220-234  ……………………….. PDF more

.

Ann Alonso Ana’s research at MSU was on metabolic flux analysis in plant central metabolism.  She was able to generate flux maps for developing seeds of sunflower and both endosperm and embryos of maize. Her findings revealed the metabolic basis of starch, protein and oil production and yielded insights into efficiency and potential metabolic engineering targets in these important tissues.  Ana also developed a method for analysing cell wall precursors and initiated flux analysis of wall synthesis in Arabidopsis cells.Ana moved to Ohio State University as a tenure-track assistant professor in 2010

Alonso AP, Piasecki RJ, Wang Y, LaClair RW, Shachar-Hill Y (2010) Quantifying the labeling and the levels of plant cell wall precursors using ion chromatography tandem mass spectrometry. Plant Physiology 153: 915-924 PDF   ……………Alonso AP, Dale VL, Shachar-Hill Y (2010) Understanding fatty acid synthesis in developing maize embryos using ……………. …………. metabolic flux analysis. Metabolic Engineering 12: 488-497 PDF …………. .. .. … …………. …… ……   ………. …………… ………. … Alonso AP, Dale VL, Shachar-Hill Y (2011) Central metabolic fluxes in the endosperm of developing maize seeds and their ………… ………. …… implications for metabolic engineering. Metabolic Engineering 13: 96-107 PDF more

.

Jean Christophe Cocuron

Christophe was our lab manager and also worked on studying metabolism in a new oilseed – Camelina. As lab manager Christophe successfully trained and supervised undergraduate assistants in the group, and significantly improved our purchasing, inventory and other administrative operations. His work on developing Camelina seeds established a culture system, determined the rates of substrate uptake and product synthesis, and intiated labeling experiments for flux analysis.Christophe moved to Ohio State University as a senior technician in 2010

.

.

Michael Watson

Mike worked, first part time as an undergraduate and then as a full time technician on culturing plant and microbial cells and analysed metabolite levels as part of metabolic flux projects.Mike moved to Phoenix to start his medical studies in May 2010

.

.

.

Becky Piasecki

Becky worked in the lab as an undergraduate research assistant. Her work included cell culturing, enzymatic and other measurements of metabolite levels. As part of our work on cell wall precursors in plant cells, Becky addressed questions about metabolite transport rates and previous work in the literature. Her dual degree studies in English came in handy when helping write a paper on the cell wall project.

 .

.

 Emily Rustad

Emily worked in the lab as an undergraduate research assistant. Her work included plant embryo culturing, and metabolite analyses. Working with Camelina embryos Emily helped define growth conditions for metabolic flux analysis. After graduating from MSU in 2011, Emily moved to California to pursue a PhD in Plant pathology at UC Riverside

.

.

.

Beth Kasiborski

Beth was  studying arbsucular mycorrhizal symbiosis and developing a system to study it in planta.

.

.

.

Other Alumni at MSU:

Postdoctoral Associates: Igor Libourel, Eva Collakova, Fernando Goffman, Heike Bucking

Technicians: Inga Krassovskaya, Ann Clements